Category Archives: Publications
High end, multi-spectral 3D tissue imaging with our C-Direct tagged anti-NCAM VHH Q55c

A recent study by Ravian van Ineveld and Michiel Kleinnijenhuis et al. in Nature Biotechnology shows the development of a new method for determining molecular, spatial and morphological features of cells in 3D tissues. The research group led by dr. Anne Rios (Princess Máxima Center for pediatric oncology, Oncode Institute) developed an eight-color, multispectral, Large-scale Single-cell […]

A novel immuno-gold labeling protocol for nanobody-based detection of HER2 in breast cancer cells using immuno-electron microscopy

Immuno-electron microscopy is commonly performed with the use of antibodies. In the last decade the antibody fragment indicated as nanobody (VHH or single domain antibody) has found its way to different applications previously done with conventional antibodies. Nanobodies can be selected to bind with high affinity and specificity to different antigens. They are small (molecular […]

Rapid optical imaging of human breast tumour xenografts using anti Her2 VHH

Abstract PURPOSE: Molecular optical imaging using monoclonal antibodies is slow with low tumour to background ratio. We used anti-HER2 VHHs conjugated to IRDye 800CW to investigate their potential as probes for rapid optical molecular imaging of HER2-positive tumours by the determination of tumour accumulation and tumour to background levels. METHODS: Three anti-HER2 VHHs (11A4, 18C3, […]

Anti adhesive VHH targeting the integrin alpha 3 beta 1 (VLA-3)

Abstract Therapeutic approaches aimed at targeting tumor surface markers using monoclonal antibodies provide a powerful strategy in cancer treatment. Here we report selection of single variable domains (VHH) of llama heavy chain antibodies, using a VHH-phage-display library. A reverse proteomic approach was used to identify the cognate proteins recognized by enriched VHH on HeLa cells. […]

Molecular Evolution of Broadly Neutralizing Llama Antibodies to the CD4-Binding Site of HIV-1

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Targeting using internalizing nanobody-decorated nanoparticles

Abstract The hepatocyte growth factor receptor (HGFR, c-Met or Met) is a receptor tyrosine kinase that is involved in embryogenesis, tissue regeneration and wound healing. Abnormal activation of this proto-oncogene product is implicated in the development, progression and metastasis of many cancers. Current therapies directed against Met, such as ligand- or, dimerization-blocking antibodies or kinase […]